Chromatography Media
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Filtered Search Results
Cytiva Capto™ Q Chromatography Media
Designed for capture and intermediate purification of proteins from large feed volumes. Cytiva CaptoTM Q Chromatography Media is a strong anion exchange BioProcess medium, designed to meet the demands of modern large-scale manufacturers.
Cytiva HiScreen™ Capto™ PlasmidSelect
For purification of supercoiled DNA to high quality, for gene therapy and DNA vaccine applications
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | Strep-Tactin XT |
| Density (Metric) | Approx. 5 mg/mL resin |
| Matrix | Rigid, cross-linked agarose, spherical |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 6 to 10 pH |
| Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
| Storage Requirements | 2 to 8°C in 20% ethanol |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media
The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process
Cytiva Capto™ MMC ImpRes Multimodal BioProcess™ Chromatography Medium
For high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules
| Particle Size | 40 μm |
|---|---|
| Ligand Type | Multimodal Weak CatIon Exchanger |
| Matrix | High-flow Agarose |
| Stability | 1 M Acetic Acid, 1 M NaOH, 2 M NaCl, 5% 1-Propanol, 30% Isopropanol, 70% Ethanol |
| Storage Buffer | 20% Ethanol, 0.2 M Sodium Acetate |
| pH Range | 3 to 12 |
| Product Type | Capto MMC ImpRes Media |
| Binding Capacity | 60 to 90 mg Mab/mL |
| Anion Exchange Capacity | 25 to 39 μM/mL |
| Storage Requirements | 4°C to 30°C |
| For Use With (Application) | For High Resolution Polishing of Monoclonal Antibodies (MAbs) and Other Biomolecules |
| Max. Pressure | 300 kPa |
Cytiva HiTrap™ Capto™ PlasmidSelect
For the purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications
| Matrix | Capto™ PlasmidSelect |
|---|---|
| Length (Metric) | 2.5 cm |
| Product Type | Chromatography Resin |
| For Use With (Application) | Purification of Supercoiled DNA From Research Scale to cGMP Production |
| Max. Pressure | 5 bar (0.5 MPa) |
Cytiva His MultiTrap™ TALON™
For high-throughput screening of histidine-tagged proteins from clarified cell lysates
Cytiva PlasmidSelect Xtra Medium
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography resin with a selectivity that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
| Particle Size | 24 to 44 μm |
|---|---|
| Ligand Type | 2-mercaptopyridine |
| Matrix | Cross-linked Agarose |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 3 to 11 |
| Product Type | PlasmidSelect Xtra Media |
| Grade | BioProcess resin |
| Storage Requirements | 4 to 30 deg.C, 20% Ethanol |
| For Use With (Application) | Generic Purification of Supercoiled Plasmid DNA |
| Max. Pressure | <120 cm/h |
Cytiva Phenyl Sepharose™ High Performance Media
Designed for intermediate and polishing step purification steps
Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media
Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.
| Particle Size | 90 μm |
|---|---|
| Ligand Type | Native Protein A (S. aureus) |
| Flow Rate | 150 to 250 cm/Hoμr |
| Matrix | 4% Cross-Linked Agarose |
| Stability | No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol |
| Storage Buffer | 20% Ethanol |
| Product Type | nProtein A Sepharose 4 Fast Flow Media |
| Binding Capacity | >35 mg Human IgG/mL |
| Height (English) Bed | 9.84 in. |
| Storage Requirements | 2°C to 8°C |
| Height (Metric) Bed | 25 cm |
| Max. Pressure | 100 kPa |
Cytiva Octyl Sepharose™ 4 Fast Flow Chromatography Media
Aliphatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification of larger proteins
| Particle Size | 45 to 165 μm |
|---|---|
| Ligand Type | Octyl |
| Matrix | 4% Cross-Linked Agarose |
| Stability | Stable in commonly used aqueous Buffers: 1 M NaOH, 30% Isopropanol, 70% Ethanol, 6 M Guanidine-Hydrochloride, 30% Acetonitrile, 1 mM HCl |
| Storage Buffer | 20% Ethanol |
| pH Range | 2 to 14 |
| Product Type | Octyl Sepharose 4 Fast Flow Media |
| Storage Requirements | 4°C to 30°C |
| Max. Pressure | 100 kPa |
Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media
Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
| Product Type | IMAC Sepharose 6 Fast Flow Media |
|---|---|
| For Use With (Application) | For Purifying Proteins and Peptides That Have an Affinity for Metal Ions |
Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media
USe for separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes.
Cytiva Butyl Sepharose™ High Performance Chromatography Media
Designed for intermediate and polishing step purification steps
| Particle Size | 24 to 44 μm |
|---|---|
| Ligand Type | Butyl |
| Flow Rate | 100 to 200 cm/Hoμr |
| Matrix | Cross-Linked Agarose |
| Stability | Stable in commonly used aqueous Buffers: 1 M Sodium Hydroxide, 1 M Acetic Acid, 8 M Urea, 6 M Guanidine Hydrochloride, 30% Acetonitrile, 70% Ethanol, 3 M Ammonium sulfate, 1 mM HCl, 30% Isopropanol, 2% SDS |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 13 |
| Product Type | Butyl Sepharose High Performance Media |
| Binding Capacity | 38 mg b-lactoglobulin/mL mediμm |
| Storage Requirements | 4°C to 30°C |
| For Use With (Application) | For Intermediate and Polishing Step PurifiCatIon Steps |
| Max. Pressure | 300 kPa |